huprottm human proteome microarrays v4.0 (Cambridge Protein Arrays)
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Huprottm Human Proteome Microarrays V4.0, supplied by Cambridge Protein Arrays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huprottm+human+proteome+microarray+v4%2E0/huprottm+human+proteome+microarrays+v4+0/pmc12041250-167-19-24
Average 90 stars, based on 1 article reviews
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1) Product Images from "Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide"
Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide
Journal: Communications Medicine
doi: 10.1038/s43856-025-00870-2
Figure Legend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.
Techniques Used: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay
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